Locus TA07186
Locus
| ID | TA07186 |
genomic context (Gviz / IGV.js)
|
| Family | TisB/IstR1 | |
| Organism | Escherichia coli str. K-12 substr. MG1655 | |
| Taxonomy | Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia coli str. K-12 substr. MG1655 | |
| Genomic location | chromosome | |
| Genomic coordinates | 3853115-3853729 | |
| Genome accession (GenBank) | NC_000913.3 | |
| Genome map | CGView | |
| Genomic context | IGV.js | |
| Upstream gene | ivbL : ilvBN operon leader peptide | |
| Within-locus gene |
istR : small regulatory RNA IstR-1 istR : small RNA IstR-2 tisB : membrane-depolarizing toxin TisB |
|
| Downstream gene | ysdE : protein YsdE |
Toxin mRNA
| Name | tisB |
predicted 2D structure (mfold / VARNA)
|
| Locus_tag | NA | |
| Genomic coordinates | 3853345-3853729 (strand +) | |
| Length | 385 nt | |
| Sequence (with 50 nt upstream, and -10 box in red; mRNA/asRNA interaction region underlined; toxin ORF in blue; transcript sequence in UPPERCASE) |
atattactgtttatttatacagtaaacttctataatatcactgtacgcaa
→ BLAST sequence
TGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGA AACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTAT GTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGG CAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGG AGACGCGTATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATT GTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATT CAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATAT TTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGAC |
|
| Predicted 2D structure | PDF, PNG |
Antitoxin asRNA
| Name | istR |
predicted 2D structure (mfold / VARNA)
|
| Locus_tag | NA | |
| Genomic coordinates | 3853115-3853198 (strand -) | |
| Length | 84 nt | |
| Sequence (with 50 nt upstream; mRNA/asRNA interaction region underlined; transcript sequence in UPPERCASE) |
acgtcaaaattcgtgccgaaattgcgcgttctgcgcggaacacgtatact
→ BLAST sequence
TTCAGTGTTGACATAATACAGTGTGCTTTGCGGTTACCAGCCGCAGGCGA CTGACGAAACCTCGCTCCGGCGGGGTTTTTTGTT |
|
| Predicted 2D structure | PDF, PNG |
Toxin Peptide
| Name | tisB |
predicted 2D structure (PSIPRED / POLYVIEW-2D)
 
hydrophobicity plot (ProtScale) 
|
| Locus_tag | NA | |
| ORF genomic coordinates | 3853553-3853642 (strand +) | |
| ORF length (peptide length) | 90 nt (29 aa) | |
| ORF sequence |
ATGAACCTGGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGC
→ BLAST sequence
ACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAA |
|
| Peptide sequence |
MNLVDIAILILKLIVAALQLLDAVLKYLK*
→ BLAST sequence
|
|
| Predicted 2D structure | PDF, GIF | |
| Hydrophobicity plot | PDF, GIF |
